IMMUNOLOCALIZATION OF LIPOXYGENASE IN PEA (PISUM-SATIVUM L) CARPELS

Polyclonal antibodies against a part of pea (Pisum sativum L.) LOXG pr otein have been raised to study the pattern of distribution of related lipoxygenases in pea carpels. The antiserum recognized three lipoxyge nase polypeptides in carpels. One of them became undetectable 24 hours after fruit development induction, suggesting that it may correspond to the protein derived from loxg cDNA. Immunolocalization experiments showed that lipoxygenase protein was present only in pod tissues: it w as abundant in the mesocarp and, from the day of anthesis, in the endo carp layers. Lipoxygenase distribution is regulated throughout develop ment. The association of lipoxygenase with cells in which processes of expansion and growth will potentially take place support a role in po d growth and development.