STUDY OF BIOCHEMICAL REACTIONS IN THIN ORGANIC FILMS BY MEANS OF EVANESCENT OPTICAL-WAVE

An optical biosensor has been developed for detection of pesticides, b ased on the evanescent optical wave technique. The concentration of th e pesticide was measured in liquid. We specially originated a core com plex of photosystem II film on a cheap disposable element. A setup on the basis of the Kretschmann arrangement was improved by using a compu ter-controlled angular scanning system. The detection concentration li mits were 10(-11) M for dinitrophenole and 10(-10) M for paraoxon. The results obtained show the perspective of the integral method for dete ction of different kinds of pesticides at extremely low concentration levels of 0.1 ppb.