M. Rucka et al., ULTRAFILTRATION MEMBRANES FROM POLYSULFONE AMINATED POLYSULFONE BLENDS WITH PROTEOLYTIC ACTIVITY/, Enzyme and microbial technology, 18(7), 1996, pp. 477-481
Ultrafiltration membranes with ''self-cleaning'' ability are presented
in this work. Porous asymmetric membranes were formed by the phase in
version method from one-to-one blends of polysulfone and its aminated
derivative. The characteristics of trypsin immobilized by static and d
ynamic sorption with cross-linking by glutaraldehyde was carried out u
sing N-alpha-benzoil-L-arginine ethyl ester (BAEE). The self-cleaning
properties are demonstrated in experiments where proteolytic membranes
were used for concentration of casein solution. The essential improve
ment of ultrafiltration flux can be observed as a result of hydrolysis
of protein blocking membrane pores.