PRODUCTION OF ENDO-1,4-BETA-GLUCANASE AND XYLANASE WITH NYLON-WEB IMMOBILIZED AND FREE TRICHODERMA-REESEI

On all media studied, the endo-1,4-beta-glucanase and xylanase activit ies obtained with the nylon-web immobilized Trichoderma reesei were hi gher than the activities with the free mycelium. The nitrogen source h ad a marked effect on endoglucanase and xylanase production on lactose , cellulose, and L-sorbose-based medium with immobilized T. reesei. Th e highest enzyme activities with this medium, 880 nkat ml(-1) for endo glucanase and 8,020 nkat ml(-1) for xylanase, were obtained in the pre sence of proteose peptone and yeast extract. Urea alone was an insuffi cient nitrogen source for enzyme production. A lactose-containing indu strial by-product-based medium offered a good alternative for endogluc anase and xylanase production. In this case, the maximum endoglucanase activity was 940 nkat ml(-1), xylanase activity was 4,410 nkat ml(-1) , and filter paper activity was 1.9 FPU ml(-1). On synthetic cellulose -based medium, the activities were 840 nkat ml(-1), 4,790 nkat ml(-1), and 3.0 FPU ml(-1), respectively. The nylon-web immobilized biocataly st could be used for two successive batch cultures with only a little decrease in enzyme production observed.