EVALUATION OF AN IMMUNOPEROXIDASE TECHNIQUE USING AN ONLY BIOTIN-LABELED ANTIBODY FOR THE DEMONSTRATION OF ACTINOBACILLUS-PLEUROPNEUMONIAE IN TISSUE-SECTIONS

In this report an immunoperoxidase technique (IPB) using an only bioti n-labeled antibody is compared to culture isolation method (CIM) for t he demonstration of Actinobacillus pleuropneumoniae serotype 1 in tiss ues of mice inoculated intravenously. The organism was isolated in 86. 7 % of mice and detected by IPB in 100% of cases. A.pleuropneumoniae a ntigen was mainly demonstrated in the liver, spleen and lungs, but als o in the kidney and brain, being especially located in the cytoplasm o f macrophages. Significant differences were observed between the resul ts obtained by both tests (P<0.001). Cross-reactions occurred by IPB w hen a rabbit anti-A.pleuropneumoniae serotype 11 serum was tested but never when an anti-serotype 9 serum was used. The immunoperoxidase tes t here described yielded much better results than CIM and it could be useful in routine diagnosis of A.pleuropneumoniae infections.