ANALYSIS OF MEMBRANE-PROTEINS OF MYCOPLASMA-CAPRICOLUM STRAINS BY SDS-PAGE AND IMMUNOBLOTTING

Sixteen moroccan and five other Mycoplasma (M.) capricolum strains wer e characterized by SDS-PAGE as well as by the immunobinding assay and immunoblotting, using antisera against M. capricolum California Kid (C K) and M. mycoides YG. There was a strong reaction of all 21 strains w ith antiserum against M. capricolum CK and of 18 strains with antiseru m against M. mycoides YG in the immunobinding assay, confirming the cr oss-reactivity between these two species observed earlier. A marked he terogeneity among the M. capricolum strains appeared in SDS-PAGE and i n immunoblotting, characterized by different protein patterns and diff erent strengths of identical protein bands. All M. capricolum strains investigated revealed, however, at least three strong protein bands wi th mol. weights of about 30, 42 and 52 KD reacting in immunoblotting w ith antiserum against M. capricolum CK but not with antiserum against M. mycoides YG. These proteins may represent antigens suitable for the identification and differentiation of M. capricolum strains.