This is the first description of a method for growing axenized Blastoc
ystis hominis as colonies in petri dishes containing soft agar. Blasto
cystis cells cultured in two types of agar appeared to show different
colonial morphologies as well as differing colony yields. Microscopic
examination of the colonies revealed many amoeboid and giant cells. Ma
ny cells were also shown to possess thin filament-like structures that
appeared to stretch across the central vacuole.