CHARACTERIZATION OF THE MCPA AND MCPB GENES CAPABLE OF ENCODING METHYL-ACCEPTING TYPE CHEMORECEPTORS IN RHODOBACTER-CAPSULATUS

Authors
MICHOTEY V TOUSSAINT B RICHAUD P VIGNAIS PM
Citation
V. Michotey et al., CHARACTERIZATION OF THE MCPA AND MCPB GENES CAPABLE OF ENCODING METHYL-ACCEPTING TYPE CHEMORECEPTORS IN RHODOBACTER-CAPSULATUS, Gene, 170(1), 1996, pp. 73-76
Citations number
21
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
170
Issue
1
Year of publication
1996
Pages
73 - 76
Database
ISI
SICI code
0378-1119(1996)170:1<73:COTMAM>2.0.ZU;2-S
Abstract
Two contiguous mcp genes, mcpA and mcpB, transcribed from the same DNA strand and capable of encoding methyl-accepting chemotaxis proteins ( Mcp) have been isolated from Rhodobacter capsulatus (Re), sequenced an d overexpressed in Escherichia coli (Ec). The deduced proteins (McpA, 69171 Da; McpB, 81629 Da) show a structure similar to that of Ec Mcp. The products of mcpA and mcpB, overproduced in Ec, were recognized by anti-Ec Mcp (Trg) antibodies.