Y. Cui et al., DEFICIENCY OF PHOSPHOTIDYLINOSITOL-LINKED MEMBRANE-PROTEINS ON ERYTHROCYTES OF DIFFERENT SUBPOPULATIONS IN PAROXYSMAL-NOCTURNAL HEMOGLOBINURIA, Chinese medical journal, 106(5), 1993, pp. 323-330
The surface phosphatidylinositol (PI)-linked proteins on membrane of p
aroxysmal nocturnal hemoglobinuria (PNH) erythrocytes (PNHE) was analy
sed by a flowcytometer (FACS 420). It was found that the loss of acety
lcholinesterase (AchE) and decay accelerating factor (DAF), two PI-lin
ked proteins, from cell membrane of PNHE was not synchronous. The hemo
lysis rates of DAF (-) and AchE (-) PNHE were much higher than that of
mixed population in cobra-venom factor (CoF) lysis test. Intact PNHE
remaining after CoF lysis had relatively lower immunofluorescent label
ing rate of AchE on membrane in comparison with normal erythrocytes. I
t implied that this subpopulation, in spite of being insensitive to co
mplement lysis, was still abormal in terms of the amount of PI-linked
protein on cell membrane. When these intact PNHE remaining after CoF l
ysis were incubated with activated polymorphonuclear leukocytes (PMN)
for three hours, immunofluorescent labeling of AchE on PNHE was promin
ently decreased. This indicated that reactive oxidants released from a
ctivated PMN might injure PI-linked proteins.