LABORATORY EVALUATION OF ACUTE UPPER GENITAL-TRACT INFECTION

Objective: To evaluate the diagnostic test characteristics of common t ests used to diagnose upper genital tract infection. Methods: Subjects included women who either met the Centers for Disease Control and Pre vention's minimal criteria for acute pelvic inflammatory disease or wh o had other signs of upper genital tract infection (ie, atypical pelvi c pain, abnormal uterine bleeding, or cervicitis). The subjects were e valuated with a baseline interview, comprehensive laboratory testing, and either an endometrial biopsy or laparoscopy for definitive diagnos is of upper genital tract infection. Patients were considered positive for upper genital tract infection if they had any of the following fi ndings: 1) histologic evidence of acute endometritis or salpingitis, 2 ) laparoscopic visualization of purulent exudate in the pelvis without another source, or 3) positive testing for Neisseria gonorrhoeae or C hlamydia trachomatis from the endometrium, fallopian tubes, or pelvis. Results: One hundred twenty women with adequate endometrial samples w ere evaluated between August 1993 and September 1995. The median age o f the study population was 24 years; 38% were white, 52% were smokers, 81% were insured by Medicaid or were uninsured, and 67% were single. Sensitivities for elevated white blood cell count (WBC), erythrocyte s edimentation rate, C-reactive protein, and increased vaginal white blo od cells are 57, 70, 71, and 78%, respectively. If any one test is abn ormal, the sensitivity is 100% and specificity is 18%. If all four tes ts are abnormal, sensitivity is 29% and specificity is 95%. Conclusion : Testing for increased vaginal white blood cells was found to be the most sensitive laboratory indicator for upper genital tract infection, whereas serum WBC was the most specific. No one diagnostic laboratory test is pathognomonic for upper genital tract infection. Combinations of positive tests can improve diagnostic specificity and positive pre dictive value, but with a diminution of sensitivity and negative predi ctive value. Combinations of negative tests can reliably exclude upper genital tract infection.