A HUMAN-CHROMOSOME-22 FOSMID RESOURCE - MAPPING AND ANALYSIS OF 96 CLONES

We have created a resource for chromosome 22 consisting of 96 unique, well-characterized Fosmids, The Fosmid vector permits efficient clonin g of DNA fragments averaging 40 kb in a single-copy vector based on th e F factor of Escherichia coli, We have found that Fosmid clones from human chromosome 22 show remarkable stability and are useful for a wid e variety of applications in genome analysis, These 96 clones have bee n localized by FISH, using high-resolution fluorescent banding and mul ticolor mapping techniques, and their position on the chromosome was c orrelated with their content of a number of common repeated sequence e lements, We identified a subset of clones likely to contain genes by r estriction analysis using the enzymes NotI, MluI, SacII, and BssHII, T his collection of cytogenetically anchored clones, representing nearly 7% of the chromosome, is of immediate value for detecting chromosomal rearrangements, for use in gene isolation, and as a framework for phy sical mapping. (C) 1996 Academic Press, Inc.