DIOLMYCINS, NEW ANTICOCCIDIAL AGENTS PRODUCED BY STREPTOMYCES SP .1. PRODUCTION, ISOLATION AND PHYSICOCHEMICAL AND BIOLOGICAL PROPERTIES

Streptomyces sp. WK-2955, a soil isolate, was found to produce a serie s of new anticoccidial compounds. Four active compounds, designated di olmycins A1, A2, B1 and B2, were isolated from the fermentation broth of the producing strain by solvent extraction, silica gel column chrom atography, gel filtration on Sephadex LH-20, and preparative HPLC. Dio lmycins inhibited the growth of Eimeria tenella in an in vitro assay s ystem using BHK-21 cells as a host. No schizont in the cells was obser ved at concentrations of 0.02 - 2.0 mug/ml for diolmycin A1, at 0.2-2. 0 mug/ml for diolmycin A2, and at 20 mug/ml for diolmycins B1 and B2.