VERY-LOW-DENSITY LIPOPROTEIN RECEPTOR BINDS APOLIPOPROTEIN E2 2 AS WELL AS APOLIPOPROTEIN E3/3/

The VLDL receptor, a newly identified lipoprotein receptor, recognizes apoE containing lipoproteins, The human VLDL receptor was overexpress ed in 1dlA-7, a mutant Chinese hamster ovary cells lacking LDL recepto rs, Each VLDL obtained from a normolipidemic subject with two epsilon 3 or epsilon 2 alleles similarly competed for the binding of radiolabe led rabbit beta-VLDL to the VLDL receptors, The anti-apoE monoclonal a ntibody 1D7, which inhibited binding of apoE3 to the LDL receptors, fa iled to compete for the binding of VLDL (apoE3 or apoE2) to the VLDL r eceptors, Results indicate that the binding site of apoE on the VLDL r eceptor may differ from its binding site on the LDL receptor.