STRUCTURES OF NEW ACIDIC O-SPECIFIC POLYSACCHARIDES OF THE BACTERIUM PROTEUS-MIRABILIS SEROGROUPS O26 AND O30

The polysaccharide chains of the lipopolysaccharides of the Proteus mi rabilis serogroups O26 and O30 were studied using sugar and methylatio n analysis and H-1 and C-13 NMR spectroscopy, including two-dimensiona l correlation spectroscopy and rotating-frame NOE spectroscopy, The po lysaccharides mere found to be acidic due to the presence of D-galactu ronic acid and its amide with L-lysine in serogroup O26 or D-glucuroni c acid in serogroup O30, and the structures of their tetrasaccharide r epeating units were established, The O26-specific polysaccharide is st ructurally and serologically related to the O-specific polysaccharide of P. Mirabilis O28, which includes amides of D-GalA with L-lysine and L-serine [Radziejewska-Lebrecht, J. et al.(1995) fur. J. Biochem. 230 , 705-712].