INDUCED OVULATION OF EUROPEAN CATFISH (SILURUS-GLANIS L) BY CARP PITUITARY, GNRH ANALOG AND OR DOPAMINE INHIBITOR ISOFLOXYTHEPIN/

Ovulation was stimulated in five groups of European catfish (5.8-19 kg bw) by intramuscular single injections of carp pituitary (5 females; CP; 4.5 mg.kg(-1) bw), or mammalian analogue of GnRH [desGly(10) (D-Al a(6)) GnRH ethylamide] (5 females; GnRHa 40 mu g.kg(-1) bw), or mammal ian analogue GnRH (GnRHa; 40 mu g.kg(-1) bw) and dopamine inhibitor (D I) Isofloxythepin (5 females; 2 mg.kg(-1) bw) or DI only (2 females; 2 mg.kg(-1) bw) in the same time at 23.0 +/- 0.12 degrees C. One group of 5 females as control was injected with Ringer solution. The best ov ulation of 80% females was found after injection of CP or GnRHa or GnR Ha with DI. The females injected with DI or Ringer solution did not ov ulate. The highest average values of ovulated ova per kg of female bw were reached in groups treated with the GnRHa preparation (13.3 +/- 3. 8%, i.e. 20493 +/- 5771 oval and CP (12.9 +/- 3.5%, i.e. 19928 +/- 543 8 ova). The value was expressively lower (8.3 +/- 4.3%, i.e. 12791 +/- 6676 oval in the group treated by the combination of GnRHa and DI, bu t these differences are not statistically significant (P > 0.05). It w as proved that the lime interval at degree hours (degrees h) between t reatment and ovulation is significantly longer (P > 0.05) when treated with the GnRHa alone (685 +/- 38 degrees h) or in combination with DI (673 +/- 41 degrees h), as compared with the CP application (482 +/- 43 degrees h). ANOVA did not show a significant effect of different tr eatments (CP, GnRHa, DI + GnRHa) on the percentage and number of ovula ted ova per kg of bw (P < 0.265).