Wavelet packet analysis was used to measure the global scaling behavio
ur of homogeneous fractal signals from the slope of decay for discrete
wavelet coefficients belonging to the adapted wavelet best basis. A n
ew scaling function for the size distribution correlation between wave
let coefficient energy magnitude and position in a sorted vector listi
ng is described in terms of a power law to estimate the Hurst exponent
. Profile irregularity and long-range correlations in self-affine syst
ems can be identified and indexed with the Hurst exponent, and synthet
ic one-dimensional fractional Brownian motion (fBm) type profiles are
used to illustrate and test the proposed wavelet packet expansion. We
also demonstrate an initial application to a biological problem concer
ning the spatial distribution of local enzyme concentration in fungal
colonies which can be modelled as a self-affine trace or an 'enzyme wa
lk'. The robustness of the wavelet approach applied to this stochastic
system is presented, and comparison is made between the wavelet packe
t method and the root-mean-square roughness and second-moment approach
es for both examples. The wavelet packet method to estimate the global
Hurst exponent appears to have similar accuracy compared with other m
ethods, but its main advantage is the extensive choice of available an
alysing wavelet filter functions for characterizing periodic and oscil
latory signals.