LIQUID-CHROMATOGRAPHIC RESOLUTION OF OPTI CAL ISOMERS - DEVELOPMENT AND CHARACTERISTICS OF CHIRAL STATIONARY PHASES CONSIST OF POLYSACCHARIDE DERIVATIVES AND APPLICATION TO LARGE-SCALE SEPARATION
A. Ohnishi et al., LIQUID-CHROMATOGRAPHIC RESOLUTION OF OPTI CAL ISOMERS - DEVELOPMENT AND CHARACTERISTICS OF CHIRAL STATIONARY PHASES CONSIST OF POLYSACCHARIDE DERIVATIVES AND APPLICATION TO LARGE-SCALE SEPARATION, Yuki Gosei Kagaku Kyokaishi, 54(5), 1996, pp. 344-353
The importance of optically active compounds for pharmaceutical usages
has increased. High-performance liquid chromatographic (HPLC) resolut
ion on chiral stationary phases (CSPs) is useful for the analysis of t
he compounds as well the sample preparations to estimate in the develo
ping stages. CSPs consisting of polysaccharide derviatives were develo
ped by adsorbing them on silica gel on the basis of information obtain
ed from cellulose triacetate. The characteristics of cellulosic esters
(OA, OB, OJ, OK) and carbamtes (OC, OF, OG, OD), and amylosic carbamt
es (AS, AD) in chiral recognition were introduced with many examples.
The optimization of separating conditions are discussed in mentioning
the differences among the acidic, neutral or basic compounds. Furtherm
ore, we introduced the principle and applications on the Simulated Mov
ing Bed process to produce optically active compounds in the industria
l scale as one of the HPLC methods.