LIQUID-CHROMATOGRAPHIC RESOLUTION OF OPTI CAL ISOMERS - DEVELOPMENT AND CHARACTERISTICS OF CHIRAL STATIONARY PHASES CONSIST OF POLYSACCHARIDE DERIVATIVES AND APPLICATION TO LARGE-SCALE SEPARATION

The importance of optically active compounds for pharmaceutical usages has increased. High-performance liquid chromatographic (HPLC) resolut ion on chiral stationary phases (CSPs) is useful for the analysis of t he compounds as well the sample preparations to estimate in the develo ping stages. CSPs consisting of polysaccharide derviatives were develo ped by adsorbing them on silica gel on the basis of information obtain ed from cellulose triacetate. The characteristics of cellulosic esters (OA, OB, OJ, OK) and carbamtes (OC, OF, OG, OD), and amylosic carbamt es (AS, AD) in chiral recognition were introduced with many examples. The optimization of separating conditions are discussed in mentioning the differences among the acidic, neutral or basic compounds. Furtherm ore, we introduced the principle and applications on the Simulated Mov ing Bed process to produce optically active compounds in the industria l scale as one of the HPLC methods.