16S rRNA genes from a range of aerobic chemoorganotrophic, alkaliphili
c soda lake Bacteria and Archaea have been sequenced and subjected to
phylogenetic analysis. Gram-negative alkaliphiles were found to be con
fined to the gamma 3 subdivision of the Proteobacteria, with many isol
ates related to the Halomonas/Deleya group. Gram-positive alkaliphiles
were found in both high % G + C and low % G C C divisions of the Gram
-positive lineage, with many isolates being related to the Bacillus gr
oup, others to Arthrobacter spp. Alkaliphilic Archaea were relatively
closely related to members of the genera Natronococcus and Natronobact
erium. An anaerobic, thermophilic isolate has been assigned to a new g
enus within the Thermotogales.