C1 BACTERIA IN THE WATER COLUMN OF CHESAPEAKE BAY, USA .3. IMMUNOLOGICAL RELATIONSHIPS OF THE TYPE SPECIES OF MARINE MONOMETHYLAMINE-OXIDIZING AND METHANE-OXIDIZING BACTERIA TO WILD ESTUARINE AND OCEANIC CULTURES

C1 bacteria oxidizing methane and monomethylamine (MMA) are readily en riched from seawater and associated marine materials, but only Methylo monas pelagica oxidizing methane and Methylophaga marina oxidizing MMA have been adequately characterized. Antisera with good specificity we re prepared against these taxonomically and trophically different ocea nic methylotrophs as well as against methanol dehydrogenase, a key enz yme presumably present in all aerobic methylotrophs. These antisera we re used in an indirect immunofluorescence procedure to compare the rel ationship of these type oceanic species to wild-type cultures from the estuarine waters of Chesapeake Bay (USA) and the open sea. Of the 32 methanotrophic enrichments attempted from the Sargasso Sea, 23 (72 %) yielded methane-oxidizers. Of the 27 isolates obtained in pure culture , 25 (96 %) were identical to Methlyomonas pelagica. By contrast, none of the 54 estuarine methane-oxidizers from Chesapeake Bay were identi cal to M pelagica, 13 % were related and 87 % were unrelated. The anti -MDH (methanol dehydrogenase) serum reacted to 23 % of the wild methan otroph cultures. Of the 18 oceanic methylaminotroph enrichments, 44 % were indistinguishable from Methylophaga marina, 6 % were related, and 50 % were unrelated. In contrast, of the 41 Chesapeake Bay MMA-oxidiz ers, 12 % were indistinguishable from M marina, 17 % were related, whi le 71 % were unrelated. The anti-MDH antiserum reacted with only 15 % of the wild MMA-oxidizing bacteria. The implications of the taxonomic affinities and trophic requirements of the methylotrophs to their estu arine and oceanic distribution and to their anaerobic methanogenic bac terial consorts are discussed.