RETINOIC ACID ENHANCES EXPRESSION OF BONE MORPHOGENETIC PROTEIN-2 IN HUMAN ADENOCARCINOMA CELL-LINE (HSG-S8)

Expression of bone morphogenetic protein (BMP)-2 mRNA was stimulated b y retinoic acid in human adenocarcinoma cell line, HSG-S8, in a dose-d ependent manner. Northern blot analysis demonstrated that retinoic aci d most strongly increased the level of BMP-2 mRNA 6 h after the treatm ent and the stimulatory effect was maintained at 48 h. The mature pept ides of 16 and 18 kDa molecular masses of BMP-2 were also increased in the conditioned medium by the treatment of retinoic acid on western b lotting. The proliferation of HSG-S8 cells was inhibited by retinoic a cid, however, retinoic acid did not cause morphological change showing cellular differentiation. 1 alpha,25(OH)(2)D-3, like retinoic acid, c learly increased the mRNA level of BMP-2 whereas dibuthryl cyclic AMP remarkably diminished it, and bromodeoxyuridine had no effect on the e xpression of BMP-2 mRNA.