A NEW APPROACH USING MULTIPLEX LONG ACCURATE PCR AND YEAST ARTIFICIALCHROMOSOMES FOR BACTERIAL CHROMOSOME MAPPING AND SEQUENCING

Citation
A. Sorokin et al., A NEW APPROACH USING MULTIPLEX LONG ACCURATE PCR AND YEAST ARTIFICIALCHROMOSOMES FOR BACTERIAL CHROMOSOME MAPPING AND SEQUENCING, PCR methods and applications, 6(5), 1996, pp. 448-453
Citations number
17
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biology
ISSN journal
10549803
Volume
6
Issue
5
Year of publication
1996
Pages
448 - 453
Database
ISI
SICI code
1054-9803(1996)6:5<448:ANAUML>2.0.ZU;2-T
Abstract
An efficient approach for structural studies on bacterial chromosomes is presented. It is based on high-resolution PCR map construction by u sing a multiplex long accurate PCR (MLA PCR) protocol and a YAC clone carrying the region to be mapped as indicator. The high-resolution PCR map of the Bacillus subtilis rmB-dnaB region is presented as an examp le. Data are also presented on the use of DNA generated by LA PCR for sequencing; they are relevant to LA PCR induced mutations and justify the application of such mapping for sequencing long stretches of bacte rial chromosomes.