QUANTAL ACETYLCHOLINE-RELEASE INDUCED BY MEDIATOPHORE TRANSFECTION

Authors
FALKVAIRANT J CORREGES P EDERCOLLI L SALEM N ROULET E BLOC A MEUNIER F LESBATS B LOCTIN F SYNGUELAKIS M ISRAEL M DUNANT Y
Citation
J. Falkvairant et al., QUANTAL ACETYLCHOLINE-RELEASE INDUCED BY MEDIATOPHORE TRANSFECTION, Proceedings of the National Academy of Sciences of the United Statesof America, 93(11), 1996, pp. 5203-5207
Citations number
26
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Proceedings of the National Academy of Sciences of the United Statesof America → ACNP
ISSN journal
00278424
Volume
93
Issue
11
Year of publication
1996
Pages
5203 - 5207
Database
ISI
SICI code
0027-8424(1996)93:11<5203:QAIBMT>2.0.ZU;2-A
Abstract
Mediatophore is a protein of approximate to 200 kDa able to translocat e acetylcholine in response to calcium. It was purified from the presy naptic plasma membranes of the electric organ nerve terminals. Mediato phore is a homooligomer of a 16-kDa subunit, homologous to the proteol ipid of V-ATPase. Cells of the N18TG-2 neuronal line are not able to p roduce quantal acetylcholine release. We show here that transfection o f N18TG-2 cells with a plasmid encoding the mediatophore subunit resto red calcium-dependent release. The essential feature of such a release was its quantal nature, similar to what is observed insitu in choline rgic synapses from which mediatophore was purified.