EXPRESSION OF A RENAL TYPE-I SODIUM PHOSPHATE TRANSPORTER (NAPI-1) INDUCES A CONDUCTANCE IN XENOPUS OOCYTES PERMEABLE FOR ORGANIC AND INORGANIC ANIONS/

Two distinct molecular types (I and II) of renal proximal tubular brus h border Na+/P-i cotransporters have been identified by expression clo ning on the basis of their capacity to induce Na+-dependent P-i influx in tracer experiments. Whereas the type II transporters (e.g., NaPi-2 and NaPi3) resemble well known characteristics of brush border Na+/P- i cotransport, little is known about the properties of the type I tran sporter (NaPi-1). In contrast to type II, type I transporters produced electrogenic transport only at high extracellular P-i concentrations (greater than or equal to 3 mM). On the other hand, expression of NaPi -1 induced a Cl- conductance in Xenopus laevis oocytes, which was inhi bited by Cl- channel blockers [5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB) > niflumic acid >> 4,4'-diisothiocyanatostilbene-2,2'-disu lfonic acid]. Further, the Cl- conductance was inhibited by the organi c anions phenol red, benzylpenicillin (penicillin G), and probenecid. These organic anions induced outwardly directed currents in the absenc e of Cl-. In tracer studies, we observed uptake of benzylpenicillin wi th a K-m of 0.22 mM; benzylpenicillin uptake was inhibited by NPPB and niflumic acid. These findings suggest that the type I Na+/P-i cotrans porter functions also as a novel type of anion channel permeable not o nly for Cl- but also for organic anions. Such an apical anion channel could serve an important role in the transport of Cl- and the excretio n of anionic xenobiotics.