ORDERED YEAST ARTIFICIAL CHROMOSOME CLONES REPRESENTING THE DICTYOSTELIUM-DISCOIDEUM GENOME

High resolution gene maps of the six chromosomes of Dictyostelium disc oideum have been generated by a combination of physical mapping techni ques. A set of yeast artificial chromosome clones has been ordered int o overlapping arrays that cover >98% of the 34-megabase pair genome. C lones were grouped and ordered according to the genes they carried, as determined by hybridization analyses with DNA fragments from several hundred genes. Congruence of the gene order within each arrangement of clones with the gene order determined from whole genome restriction s ite mapping indicates that a high degree of confidence can be placed o n the clone map. This clone-based description of the Dictyostelium chr omosomes should be useful for the physical mapping and subcloning of n ew genes and should facilitate more detailed analyses of this genome.