IMPROVED DETECTION OF HUMAN PAPILLOMAVIRUS INFECTION IN GENITAL INTRAEPITHELIAL NEOPLASIA IN HUMAN-IMMUNODEFICIENCY-VIRUS POSITIVE (HIV-REACTION IN-SITU HYBRIDIZATION() WOMEN BY POLYMERASE CHAIN)
F. Walker et al., IMPROVED DETECTION OF HUMAN PAPILLOMAVIRUS INFECTION IN GENITAL INTRAEPITHELIAL NEOPLASIA IN HUMAN-IMMUNODEFICIENCY-VIRUS POSITIVE (HIV-REACTION IN-SITU HYBRIDIZATION() WOMEN BY POLYMERASE CHAIN), Diagnostic molecular pathology, 5(2), 1996, pp. 136-146
The prevalence of genital human papillomavirus (HPV) infection was eva
luated in 30 consecutive human immunodeficiency virus (HIV)+ women by
polymerase chain reaction (PCR)-in situ hybridization (ISH) on paraffi
n-embedded tissue sections and compared with that found with standard
ISH. Biopsies were removed from normal or neoplastic areas in the cerv
ix, vagina, and vulva, and ISH was performed with biotinylated or fluo
rescein isothiocyanate genomic DNA probes. One probe was used for HPV
screening and others for HPV typing (types 6, 11, 16, 18, 31, and 33).
Sequences were amplified by the ''hot-start'' PCR method and followed
by standard ISH. Among the 30 HIV+ women, 90% scored HPV+ in one or s
everal locations by PCR-ISH, whereas only 67% were positive by ISH. On
cogenic HPV types were found in 63% by PCR-ISH and in only 43% by ISH.
The same HPV types detected by standard ISH were also recognized by P
CR-ISH, but with the latter the signal was amplified. Moreover, some H
PV types were found with PCR-ISH but not by ISH. We conclude that PCR-
ISH is a valuable and sensitive method for specific detection of HPV.