PRODUCTION OF CELLULASE AND BETA-GLUCOSIDASE ACTIVITY DURING GROWTH OF THE ACTINOMYCETE MICROMONOSPORA-CHALCAE ON CELLULOSE-CONTAINING MEDIA

Authors
GALLAGHER J WINTERS A BARRON N MCHALE L MCHALE AP
Citation
J. Gallagher et al., PRODUCTION OF CELLULASE AND BETA-GLUCOSIDASE ACTIVITY DURING GROWTH OF THE ACTINOMYCETE MICROMONOSPORA-CHALCAE ON CELLULOSE-CONTAINING MEDIA, Biotechnology letters, 18(5), 1996, pp. 537-540
Citations number
10
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
18
Issue
5
Year of publication
1996
Pages
537 - 540
Database
ISI
SICI code
0141-5492(1996)18:5<537:POCABA>2.0.ZU;2-V
Abstract
The actinomycete, Micromonospora chalcae produced an extracellular cel lulase system when grown on cellulose-containing media. Cellulase acti vity reached a maximum after 8 days and the beta-glucosidase activity was maximal within 16 to 18 days. beta-Glucosidase activity was isolat ed and had a Km of 0.25mM for the substrate p-nitrophenyl-beta-D-gluco side. The activity was optimal at pH 7.25 and 52 degrees C.