SYNTHESIS OF SULFATED BIOACTIVE PEPTIDES USING IMMOBILIZED ARYLSULFOTRANSFERASE FROM EUBACTERIUM SP

Authors
DELHOM B ALVARO G CAMINAL G TORRES JL CLAPES P
Citation
B. Delhom et al., SYNTHESIS OF SULFATED BIOACTIVE PEPTIDES USING IMMOBILIZED ARYLSULFOTRANSFERASE FROM EUBACTERIUM SP, Biotechnology letters, 18(5), 1996, pp. 609-614
Citations number
18
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
18
Issue
5
Year of publication
1996
Pages
609 - 614
Database
ISI
SICI code
0141-5492(1996)18:5<609:SOSBPU>2.0.ZU;2-F
Abstract
An arylsulfotransferase from Eubacterium sp. was immobilized on agaros e gels by multipoint covalent attachment. The yield of immobilization was 80% with an activity of 11 UA/ml of derivative. After 19 days of i ncubation, the loss of activity of the derivative was 36%. The immobil ized preparation was used to transfer selectively a sulfate group from p-nitrophenolsulfate to selected tyrosine containing biologically act ive peptides in 92-99% of yield.