REVIEW - THE CCT EUKARYOTIC CHAPERONIN SUBUNITS OF SACCHAROMYCES-CEREVISIAE AND OTHER YEASTS

All eight of the CCT1-CCT8 genes encoding the subunits of the Cct chap eronin complex in Saccharomyces cerevisiae have been identified, inclu ding three that were uncovered by the systematic sequencing of the yea st genome. Although most of the properties of the eukaryotic Cct chape ronin have been elucidated with mammalian systems in vitro, studies wi th S. cerevisiae conditional mutants revealed that Cct is required for assembly of microtubules and actin in vivo. Cct subunits from the oth er yeasts, Candida albicans and Schizosaccharomyces pombe, also have b een identified from partial and complete DNA sequencing of genes. Cct8 p from C. albicans, the only other completely sequenced Cct protein fr om a fungal species other than S. cerevisiae, is 72% and 61% similar t o the S. cerevisiae and mouse Cct8 proteins, respectively. The C. albi cans CCT8 sequence has been assigned the Accession Number U37371 in th e GenBank/EMBL database.