SEQUENCE-ANALYSIS OF A 14.2 KB FRAGMENT OF SACCHAROMYCES-CEREVISIAE CHROMOSOME-XIV THAT INCLUDES THE YPT53, TRNA(LEU) AND GSR M2 GENES AND 4 NEW OPEN READING FRAMES

As part of the EU yeast genome program, a fragment of 14 262 bp from t he left arm of Saccharomyces cerevisiae chromosome XIV has been sequen ced. This fragment corresponds to cosmid 14-14b and is located roughly 130 kb from the centromere. It contains four new open reading frames which encode potential proteins of more than 99 amino acids, as well a s the ypt53, tRNA(Leu) and gsr m2 genes. The putative protein N2212 is similar to the ribosomal protein S7 from humans. N2215 contains sever al predicted transmembrane elements. N2231 contains regions which are rich in acidic, as well as basic, residues which could form a-helical structures. Similar regions are found in a variety of proteins includi ng glutamic acid rich protein, trichohyalin, caldesmon, Tb-29 and seve ral cytoskeleton-interacting proteins. The sequence has been entered i n the EMBL data library under Accession Number X85811.