SYNTHESIS AND APPLICATION OF BORONIC ACID-IMMOBILIZED POROUS POLYMER PARTICLES - A NOVEL PACKING FOR HIGH-PERFORMANCE LIQUID AFFINITY-CHROMATOGRAPHY

A preparation method of a novel type of packing materials for high-per formance liquid affinity chromatography to determine glycated proteins was studied, and a fundamental study on its application to diabetic s erum was conducted. To quantify glycated proteins such as glycated ser um albumin, a new hydrophilic and durable porous polymer particle rece ntly developed in our laboratory was used as the basic matrix. The mat rix was activated with 1,1'-carbonyldiimidazole (CDI), and optimizatio n of the coupling reaction between these CDI-activated matrix and m-am inophenylboronic acid hemisulfate (APBA) as affinity ligand was invest igated. The optimum value for the APBA coupling yield was found to be obtained under acidic conditions very different from the data reported by previous workers. Using this APBA-immobilized matrix an affinity c olumn was prepared, and its usefulness in HPLC separation of glycated serum proteins was investigated. Also, the fundamental and preliminary results for diagnosis of diabetes mellitus are discussed in this pape r.