DETERMINATION OF AMPHETAMINE AND RELATED-COMPOUNDS IN URINE USING ONLINE DERIVATIZATION IN OCTADECYL SILICA COLUMNS WITH 9-FLUORENYLMETHYL CHLOROFORMATE AND LIQUID-CHROMATOGRAPHY
R. Herraezhernandez et al., DETERMINATION OF AMPHETAMINE AND RELATED-COMPOUNDS IN URINE USING ONLINE DERIVATIZATION IN OCTADECYL SILICA COLUMNS WITH 9-FLUORENYLMETHYL CHLOROFORMATE AND LIQUID-CHROMATOGRAPHY, Journal of chromatography B. Biomedical applications, 679(1-2), 1996, pp. 69-78
Citations number
19
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
A method for the determination of amphetamine and related compounds in
urine based on on-line derivatization with 9-fluorenylmethyl chlorofo
rmate (FMOC) and high-performance liquid chromatography is described.
Derivatization is performed in a 20 x 2.1 mm I.D. column packed with a
Hypersil ODS C-18, 30 mu m stationary phase, which is also used for s
ample clean-up and enrichment of the analytes. Next, the derivatized a
nalytes are transferred to a LiChrospher 100 RP-C-18 (5 mu m, 125 x 4
mm I.D.) analytical column for their separation and quantification, us
ing reversed-phase conditions and fluorescence detection. The describe
d assay was applied to the determination of norephedrine, ephedrine, p
seudoephedrine, amphetamine, phenylpropylamine and methamphetamine at
concentrations of 0.5-10.0 mu g/ml. Analyte conversions were about 55-
96% of those obtained by the off-line derivatization mode under simila
r conditions, resulting in limits of detection in the 5-25 ng/ml range
.