THE EFFECT OF 6-DIMETHYLAMINOPURINE (6-DMAP) ON DNA-SYNTHESIS IN ACTIVATED MAMMALIAN OOCYTES

Authors
LEDDA S LOI P BOGLIOLO L MOOR RM FULKA J
Citation
S. Ledda et al., THE EFFECT OF 6-DIMETHYLAMINOPURINE (6-DMAP) ON DNA-SYNTHESIS IN ACTIVATED MAMMALIAN OOCYTES, Zygote, 4(1), 1996, pp. 7-9
Citations number
12
Categorie Soggetti
Developmental Biology","Cell Biology
Journal title
Zygote → ACNP
ISSN journal
09671994
Volume
4
Issue
1
Year of publication
1996
Pages
7 - 9
Database
ISI
SICI code
0967-1994(1996)4:1<7:TEO6(O>2.0.ZU;2-2
Abstract
Mouse, sheep and bovine metaphase II oocytes were briefly preincubated in medium with 6-dimethylaminopurine (6-DMAP) and thereafter activate d with ethanol before further culture in 6-DMAP supplemented medium. T he presence of 6-DMAP enhances the efficiency of activation and the sp eed of pronuclear formation but has no effect on DNA synthesis. These results are discussed in the context of recently published data showin g that the preincubation of condensed chromosomes with 6-DMAP blocks D NA synthesis when nuclei become reformed.