MEASUREMENT OF TOTAL SERUM IGE BY ELISA U SING 3 COMMERCIAL KITS

We measured total serum IgE in 14 patients with allergic diseases and 16 healthy subjects, using three commercial ELISA kits. The correlatio n of results among the three kits was analyzed using Passing and Bablo ck regression parameters. Results show that measurements of the differ ent kits do not coincide. One kit shows differences using sera from no rmal subjects. There is no correlation among kits when using sera from allergic patients. It is concluded that it is not possible to determi ne exactly the amount of IgE using these kits, specially in subjects w ith elevated levels.