METHOD FOR THE ANALYSIS OF S9788, A DRUG TO REVERSE RESISTANCE TO ANTICANCER AGENTS, IN ANIMAL PLASMA AND HUMAN PLASMA AND SERUM BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION

An extraction method has been developed using benzene sulphonyl cation -exchange sample preparation cartridges and reversed-phase high-perfor mance liquid chromatography with ultraviolet detection for the measure ment of S9788, a drug to reverse resistance to anticancer agents, in p lasma and serum. This includes a toxicokinetic assay which has a mean precision and accuracy of 11.7% and 7.9%, respectively, over the range 10-1000 ng ml-1 and a quantification limit of 10 ng ml-1 and a more s ensitive pharmacokinetic procedure with a mean precision and accuracy of 5.0% and 7.9%, respectively, over the range 1-500 ng ml-1 and a qua ntification limit of 1 ng ml-1. The specificity of the procedure has b een demonstrated by mass and ultraviolet spectrometry, and linearity, precision, accuracy, recovery and sensitivity have been established. T he assays have been successfully applied to toxicokinetic and pharmaco kinetic studies.