ENANTIOSELECTIVE ASSAY OF WARFARIN IN BLOOD-PLASMA BY LIQUID-CHROMATOGRAPHY ON CHIRALCEL OC

Authors
ANDERSEN C BALMER K LAGERSTROM PO
Citation
C. Andersen et al., ENANTIOSELECTIVE ASSAY OF WARFARIN IN BLOOD-PLASMA BY LIQUID-CHROMATOGRAPHY ON CHIRALCEL OC, Journal of chromatography. Biomedical applications, 615(1), 1993, pp. 159-163
Citations number
9
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography. Biomedical applications → ACNP
ISSN journal
03784347
Volume
615
Issue
1
Year of publication
1993
Pages
159 - 163
Database
ISI
SICI code
0378-4347(1993)615:1<159:EAOWIB>2.0.ZU;2-X
Abstract
A stereoselective method for the two enantiomers of warfarin has been developed. Warfarin is extracted into dichloromethane-hexane from bloo d plasma. After concentration by evaporation, the enantiomers are reso lved by liquid chromatography on Chiralcel OC employing a non-polar mo bile phase. By fluorometric detection, concentrations in plasma down t o 80 nmol/l (25 ng/ml) can be determined with a coefficient of variati on of less than 15%. At the 200 nmol/l level, a coefficient of variati on of ca. 4% was found.