HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF MORPHINE ANDITS METABOLITES IN PLASMA USING DIODE-ARRAY DETECTION

Authors
WIELBO D BHAT R CHARI G VIDYASAGAR D TEBBETT IR GULATI A
Citation
D. Wielbo et al., HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF MORPHINE ANDITS METABOLITES IN PLASMA USING DIODE-ARRAY DETECTION, Journal of chromatography. Biomedical applications, 615(1), 1993, pp. 164-168
Citations number
14
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography. Biomedical applications → ACNP
ISSN journal
03784347
Volume
615
Issue
1
Year of publication
1993
Pages
164 - 168
Database
ISI
SICI code
0378-4347(1993)615:1<164:HLDOMA>2.0.ZU;2-X
Abstract
An isocratic high-performance liquid chromatographic method has been d eveloped for the determination of morphine, codeine, normorphine, morp hine 3-glucuronide and morphine 6-glucuronide in plasma using a diol c olumn and diode-array detection. Samples were extracted using solid-ph ase extraction with recoveries in excess of 90%. The limit of determin ation was 1 ng/ml for morphine, codeine and morphine 3-glucuronide, an d 10 ng/ml for normorphine and morphine 6-glucuronide. Inter- and intr a-day precision were better than 10%.