THE USE OF AUTOMATED SOLID-PHASE EXTRACTION IN THE 96-WELL FORMAT FORHIGH-THROUGHPUT BIOANALYSIS USING LIPID CHROMATOGRAPHY COUPLED TO TANDEM MASS-SPECTROMETRY

Authors
ALLANSON JP BIDDLECOMBE RA JONES AE PLEASANCE S
Citation
Jp. Allanson et al., THE USE OF AUTOMATED SOLID-PHASE EXTRACTION IN THE 96-WELL FORMAT FORHIGH-THROUGHPUT BIOANALYSIS USING LIPID CHROMATOGRAPHY COUPLED TO TANDEM MASS-SPECTROMETRY, Rapid communications in mass spectrometry, 10(7), 1996, pp. 811-816
Citations number
8
Categorie Soggetti
Spectroscopy,"Chemistry Analytical
Journal title
Rapid communications in mass spectrometry → ACNP
ISSN journal
09514198
Volume
10
Issue
7
Year of publication
1996
Pages
811 - 816
Database
ISI
SICI code
0951-4198(1996)10:7<811:TUOASE>2.0.ZU;2-W
Abstract
A high throughput mass spectrometry based method is described for the determination of the 5-HT receptor agonist 311C90, and its desmethyl m etabolite, in human plasma, Samples were extracted using the MicroLute (TM) system of solid phase extraction in the '96 well' format, automat ed by means of a robotic sample processor The extracts were analysed b y liquid chromatography tandem mass spectrometry (LC/MS/MS) with therm ally assisted electrospray ionization (TurboIonSpray) and selected-rea ction monitoring, The LC/MS/MS method offers increased sensitivity, se lectivity and speed of analysis over an existing high performance liqu id chromotographic method using fluorescence detection.