A. Merighi et al., QUALITY IMPROVEMENT IN GASTROINTESTINAL ENDOSCOPY - MICROBIOLOGIC SURVEILLANCE OF DISINFECTION, Gastrointestinal endoscopy, 43(5), 1996, pp. 457-462
Background: Cleaning and disinfection procedures play an essential rol
e in the prevention of infection transmission in gastrointestinal endo
scopy. In spite of published detailed guidelines, several variants and
weak points still exist. Methods: Cleaning-disinfection procedures we
re carried out according to ''Working Party, Sydney 1990.'' A microbio
logic surveillance protocol tested the contamination of endoscopes and
of automatic washing machines. To assess and improve the efficacy of
disinfection, we adopted a quality assurance program. Results: During
a 2-year follow-up, the outside surfaces of gastroscopes were contamin
ated in 60.5% and channels in 41.3%; the outside areas of colonoscopes
were contaminated in 62.3% and channels in 40.3%. Isolated bacteria w
ere gram-negative organisms, particularly Pseudomonas species, and gra
mpositive organisms, mostly Staphylococcus species. The water reservoi
rs of automatic washing machines were frequently contaminated by Pseud
omonas aeruginosa. The disinfection of washing machines and alcohol ri
nsing of endoscopes after standard procedures significantly reduced th
e bacterial contamination. Conclusions: The microbiologic surveillance
pointed out the main weak points that could be improved by the adopti
on of corrective interventions. Quality assurance is a feasible method
to assess the efficacy of cleaning-disinfection, and its wide applica
tion would improve quality of care.