ELECTROCHEMILUMINESCENCE DETECTION OF GLUCOSE-OXIDASE AS A MODEL FOR FLOW-INJECTION IMMUNOASSAYS

Glucose oxidase was covalently attached to aminosilanized indium tin o xide coated glass wafers and these were used as working electrodes in an electrochemiluminescence flow injection analyser as a model for enz yme immunoassays. Light from the chemiluminescent reaction between enz ymatically produced hydrogen peroxide and electrochemically oxidized l uminol was detected and the effect of pH and luminol concentration on this reaction was determined. Repetitive assays for glucose were carri ed out to investigate the surface chemistry. Results suggested that el ectrochemiluminescence enzyme immunoassays with glucose oxidase as the antibody label could be carried out. (C) 1996 Elsevier Science Limite d