The generator of sinusoidal signals of substrate concentration was dev
eloped using two sets of twin cylinders. The generator can generate th
e sinusoidal wave of substrate concentration of required frequency and
amplitude by the adjustment of feeding substrate concentration and of
the frequency and stroke of reciprocal motion of plungers. The perfor
mance characteristics of the generator developed were investigated. Th
e generator was applied to the study of frequency response of a glucos
e sensor based on immobilized glucose oxidase membrane. The frequency
response of the glucose sensor was modeled, as the first approximation
, by the first order system with dead time. (C) 1996 Elsevier Science
Limited