We compared the genomes of nine dog rabies virus isolates using two mo
lecular methods. The viruses used in the comparison included three Eth
iopian rabies strains from carrier dogs, a street strain from a rabid
dog from the same geographic area, two saliva isolates made from an ex
perimentally infected carrier dog, the virus isolated from the tonsil
of this carrier dog at necropsy, and two laboratory strains. We produc
ed overlapping polymerase chain reaction (PCR) segments spanning 97% o
f the genome. Restriction analysis of these PCR products with AvaII, B
ell, and BamHI detected 39 variable sites representing 668 nucleotides
(nt) or 5.5% of the genome. We also compared the DNA and the deduced
peptide sequences of a 200-nt segment of the 3' end of the rabies nucl
eoprotein gene. Previous work with these Ethiopian carrier viruses and
the endemic street strain had failed to show any differences among th
em. Both restriction mapping and sequence analysis of 200 nt of the nu
cleoprotein gene allowed us to individually identify these isolates. P
hylogenetic analyses of these data sets showed only the two saliva iso
lates of the experimentally infected carrier dog to be identical. Each
of the viruses in this study, including the one isolated from the ton
sil of the experimentally infected carrier dog, could be distinguished
by these techniques.