Live eosinophils when mixed with Acridine Orange solution and viewed m
icroscopically using u.v. light show very intense colours of their gra
nules (yellow, orange and red) and green nuclear staining. Their activ
e movement, translocation of granules and degranulation can be observe
d in vitro. Using this method, live eosinophils can be easily differen
tiated and enumerated. Copyright (C) 1996 Australian Society for Paras
itology.