GLUCOSE-INDUCED DECREASE IN GLUTAMATE LEVELS IN ISCHEMIC HUMAN BRAIN BY IN-VIVO MICRODIALYSIS

Glutamate is one of the principal neurotoxins in the pathogenesis of i schemic neuronal injury. Elevated glutamate levels in ischemia have be en well documented in many animal stroke models. Recent work in humans also shows a similar trend. We have used our acute focal ischemic mod el of the human brain to study the response of glutamate levels by in vivo microdialysis during ischemia using two different perfusates. The addition of 30 mM of glucose to the perfusate attenuated the percenta ges of dialysate glutamate levels from 4.27 +/- 1.7 to 1.34 +/- 0.47 ( P < 0.001) during partial ischemia and from 21.42 +/- 6.05 to 7.25 +/- 1.43 (P < 0.05) with total ischemia. The pre-ischemic values of gluta mate were similar with the two perfusates These results indicate that the ischemia-induced rise in glutamate is attenuated by exogenous gluc ose delivery in the human stroke model.