CHRONIC EXPOSURE OF NEONATAL CARDIAC MYOCYTES TO HYDROGEN-PEROXIDE ENHANCES THE EXPRESSION OF CATALASE

The adaptive response of antioxidant enzymes to oxidative stress in th e heart has not been investigated. Because H2O2 is considered to play a major role in reperfusion injury to the heart, this study was undert aken to examine if H2O2 alters the expression of these enzymes in the rat neonatal myocytes. H2O2 was continuously generated by the addition of glucose oxidase to the culture medium. Both the activity and the m RNA for catalase were increased following incubation of neonatal myocy tes with non-toxic concentrations of glucose oxidase. The induction of mRNA preceded the enhancement of activity, and both remained elevated after 24 h incubation. Nuclear run-on assay indicated that the transc riptional rate for catalase mRNA was increased. The same treatment did not alter the activities for total superoxide dismutase and Se-glutat hione peroxidase. The results suggest that H2O2-induced expression of catalase was in part due to transcriptional activation. It further imp lies that the induction of catalase may play an important role in prot ecting hearts from ischemic/reperfusion injury. (C) 1996 Academic Pres s Limited