GRADIENT LIQUID-CHROMATOGRAPHIC METHOD FOR DETERMINATION OF CHLORHEXIDINE AND ITS DEGRADATION PRODUCTS IN BULK MATERIAL

A liquid chromatographic method was developed for determination of chl orhexidine and its degradation products in unformulated drug substance . A nonlinear gradient from 80% 0.1 M ammonium acetate buffer, pH 5.0, to 20% buffer over 90 min (balance is acetonitrile) is applied to a 3 mu m octadecylsilane bonded-phase column. The drug and some of its de gradation products are determined at 230 nm. Of 11 previously identifi ed degradation products, 9 are determined with good precision (relativ e standard deviation of peak area is <2%).