PROCOAGULANT ACTIVITY OF MONONUCLEAR-CELLS IS INCREASED IN MYELOPROLIFERATIVE AND MYELODYSPLASTIC DISEASES

Procoagulant activity (PCA) of peripheral mononuclear cells (PMC) was evaluated in patients with primary thrombocythemia (PT, group A), poly cythemia vera (PV), idiopathic myelofibrosis (IM) and myelodysplastic syndromes (group B), and in 15 healthy subjects as control group. PCA of PMC was assayed under basal conditions and after agonist-induced st imulation: bacterial lipopolysaccharide, glycosylated granulocyte-macr ophage colony-stimulating factor, recombinant alpha-interferon. PCA wa s similar in the control group and group A when no stimulation was use d, while PCA was found significantly higher in group B patients in the same conditions. In group A patients and in the control group, but no t in group B patients, a lower PCA expression was found when PMC were simultaneously coincubated with LPS and alpha-interferon with respect to LPS incubation alone.