G. Alemany et al., SIMULTANEOUS DETERMINATION OF VANILLYLMANDELIC, HOMOVANILLIC AND 5-HYDROXYINDOLEACETIC ACIDS IN HUMAN URINE BY THIN-LAYER CHROMATOGRAPHY, BMC. Biomedical chromatography, 10(3), 1996, pp. 144-145
Citations number
7
Categorie Soggetti
Chemistry Analytical","Pharmacology & Pharmacy",Biology,"Biochemical Research Methods
A TLC method for the simultaneous analysis of vanillylmandelic, homova
nillic and 5-hydroxyindole-3-acetic acids in urine is described, The s
ample is cleaned up through a cyano minicolumn and extracted with diet
hyl ether, The acids are resolved by high-performance TLC, visualized
by Folin Ciocalteau reagent and quantificated by densitometry at 600 n
m with beta-(4-hydroxy-3-phenyl) acetic acid as the internal standard.