ESTABLISHMENT AND CHARACTERIZATION OF A NEW CANINE B-CELL LEUKEMIA-CELL LINE

A new cell line derived from a spontaneous canine leukemia was establi shed and designated GL-1. The cells have been cultured in a floating f ashion and passaged for over two years. They were round with rich cyto plasm containing many rough endoplasmic reticula and mitochondria. Per oxidase staining was negative. The nuclei of many cells were round, bu t segmented nuclei were seen frequently. The doubling time of the cell s was 27.3 hr and they had 78 chromosomes. Surface marker analysis usi ng monoclonal antibodies (MABs) and flowcytometry revealed that GL-1 p ossessed CD45 and surface IgG. However, the cells did not react with M ABs detecting T-cell markers. These results indicate that GL-1 has a l ymphocytic lineage and is derived from a B-cell leukemia.