INTEGRATED GENETIC-MAP OF ANOPHELES-GAMBIAE - USE OF RAPD POLYMORPHISMS FOR GENETIC, CYTOGENETIC AND STS LANDMARKS

Randomly amplified polymorphic DNA (RXPD) markers have been integrated in the genetic and cytogenetic maps of the malaria vector mosquito, A nopheles gambiae. Fifteen of these markers were mapped by recombinatio n, relative to microsatellite markers that had been mapped previously. Thirty-four gel-purified RAPD bands were cloned and sequenced, genera ting sequence tagged sites (STSs) that can be used as entry points to the A. gambiae genome. Thirty one of these STSs were localized on nurs e cell polytene chromosomes through their unique hybridization signal in in situ hybridization experiments. Five STSs map close to the break points of polymorphic inversions, which are notable features of the An opheles genome. The usefulness and limitations of this integrated mosq uito map are discussed.