G. Hou et al., RELATIONSHIPS OF QUANTITY OF GLIADIN SUBGROUPS OF SELECTED US SOFT WHEAT FLOURS TO RHEOLOGICAL AND BAKING PROPERTIES, Cereal chemistry, 73(3), 1996, pp. 352-357
The gliadin subgroups (omega-, gamma-, beta-, and alpha-gliadins) in 1
7 soft wheat patent flours from four wheat classes and seven straight-
grade flours were identified and quantified by acid polyacrylamide gel
electrophoresis (A-PAGE) coupled with densitometry using a known quan
tity of modified Osborne gliadins as a quantitative standard. Flour rh
eological properties were evaluated by alveograph, farinograph, and mi
xograph tests. Japanese-type sponge cakes (JSC) and AACC sugar-snap co
okies (SSC) were made to evaluate the flours' baking performances. The
results showed that the quantity of cr-gliadins was wheat class relat
ed. The percentages of individual gliadin subgroups in flour protein w
ere significantly lower in the patent flours than in counterpart strai
ght-grade flours. The quantities of certain gliadin subgroups and tota
l gliadins have various associations with flour rheological properties
, and JSC and SSC end-use quality for each class of wheat.